https://eln.excellgen.com/index.php?action=history&feed=atom&title=1236_pCDNA3.1-AvPAL-2 2026-10-18T10:35:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1236_pCDNA3.1-AvPAL-2&diff=4618&oldid=prev 2012-06-13T15:54:11Z

<p></p> <p><b>New page</b></p><div>= pCDNA3.1-AvPAL-2&lt;br&gt; =<br /> <br /> &#039;&#039;&#039;Date: 2012-6-10&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Page&#039;&#039;&#039;:&amp;nbsp; &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; 1236 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; &lt;span style=&quot;font-weight: bold;&quot;&gt;Paul&lt;/span&gt;&lt;br&gt; <br /> <br /> == Cloning ==<br /> <br /> 6/10/2012. Synthesize fragment 2 using KAPA, 20 cycles.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1236 1219 6 10 2012.jpg]]&lt;br&gt; <br /> <br /> digest PCR product with BamH1+ EcoR1&lt;br&gt; <br /> <br /> [[Image:1236_1219_digestion_6_10_2012.jpg]]<br /> <br /> digest pCDNA3.1 with BamH1+ EcoR1, ligate and transform Top10&lt;br&gt; <br /> <br /> == Colony Screening by Restriction Digestion&lt;br&gt; ==<br /> <br /> [[Image:1236 Digestion.JPG]] <br /> <br /> Digested samples with BamHI and EcoRI, positive in samples 1, 3, 4, 5 and 6. <br /> <br /> Submitted sample 5 for sequencing.This clone is good. Make glycerol stock&lt;br&gt;<br /> <br /> [[Category:Cloning]]</div>

Paul.xu