https://eln.excellgen.com/index.php?action=history&feed=atom&title=1238_pCDNA3.1-DEST 2026-10-18T02:24:37Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1238_pCDNA3.1-DEST&diff=5922&oldid=prev 2012-09-05T20:24:35Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of&amp;nbsp;pCDNA3.1-DEST&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of&amp;nbsp;pCDNA3.1-DEST <br /> <br /> &#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; 06/12/2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1238 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; Paul Xu&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Colony Screening&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> Date:&amp;nbsp;06/12/2012&lt;br&gt; <br /> <br /> [[Image:1238 Digestion.JPG]] <br /> <br /> Digested with&amp;nbsp;Kpn1 + Xho1, inconclusive results (supposed to be ~1.7 kb) <br /> <br /> &lt;br&gt; <br /> <br /> 6/15/2012 <br /> <br /> digest with Kpn1 + Xho1 <br /> <br /> [[Image:1235 1238 1933 digestion 6 15 2012.jpg]] <br /> <br /> [[Image:1235 1238 1933 digestion2 6 15 2012.jpg]] <br /> <br /> [[Image:1235 1238 1023 6 18 2012.jpg]] <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Purpose: Cloning of pCDNA3.1-DEST <br /> <br /> &lt;br&gt; <br /> <br /> Date: 06/20/2012 <br /> <br /> Notebook: <br /> <br /> Page: <br /> <br /> Clone ID: 1238 <br /> <br /> Experimenters: yingying lu&lt;br&gt; <br /> <br /> [[Image:1235+1238 clone 6-21-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | lane 3&lt;br&gt; <br /> | lane 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 374 pAd5-CMV-Dest-C6XHis&lt;br&gt; <br /> | 1226 pCDNA3.1&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Kpn1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> 2012-6-25&lt;br&gt; <br /> <br /> digest detect:Kpn1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1238 digest detect 6-25-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> 2012-6-26 <br /> <br /> 1238-clone5 digest detect <br /> <br /> [[Image:1235+1238 digest 6-26-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> 2012-6-29 <br /> <br /> band 1 <br /> <br /> 1238-5 digest&amp;nbsp;:BamH1 <br /> <br /> [[Image:1238 digest detect 6-29-2012.JPG]] <br /> <br /> <br /> <br /> <br /> <br /> gateway reaction<br /> <br /> 2012-9-5<br /> <br /> [[Image:1256+1341_digest_detct_9-5-2012.JPG]]<br /> <br /> [[Category:Cloning]]</div>

Paul.xu