https://eln.excellgen.com/index.php?action=history&feed=atom&title=1240_pET28b-3C 1240 pET28b-3C - Revision history 2026-10-18T11:51:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1240_pET28b-3C&diff=7556&oldid=prev Paul.xu at 18:18, 17 December 2012 2012-12-17T18:18:54Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1240 pET28b-3C&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1240 pET28b-3C <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Dec, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1240 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> gene synthesis <br /> <br /> [[Image:1240 549-1 549-2PCR.JPG]]&lt;br&gt; digest pcr products with Nde1 + Xho1 for 2 hours. diget pET28b with Nde1 + Xho1 for 1.5 hour. <br /> <br /> [[Image:1118PCR 1422 1240PCR V12 1505DIGEST.JPG]]<br /> <br /> screen<br /> <br /> [[Image:1118_1240digest.JPG]]<br /> <br /> [[Category:Cloning]] <br /> <br /> [[Category:Cloning]]</div> Paul.xu