https://eln.excellgen.com/index.php?action=history&feed=atom&title=1245_pDB.His.MBP.scIHF2 2026-10-18T05:48:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1245_pDB.His.MBP.scIHF2&diff=5008&oldid=prev 2012-07-09T15:56:35Z

<p><span class="autocomment">Colony Screening</span></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1245 pDB.His.MBP.scIHF2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1245 pDB.His.MBP.scIHF2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;July 6, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1245 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Amplified from 1078 template with scIHF-Nhel-F and scIHP-2-16 primers, expected size 620 bp <br /> <br /> Cut out band from lane 3, purified digested with 1217 vector with RE&amp;nbsp;Nhe1 and Xho1 <br /> <br /> [[Image:0706 amp.JPG]] <br /> <br /> Digested DNA&amp;nbsp;with 1217 vector with RE Nhe1 and Xho1 &lt;br&gt; <br /> <br /> Cut out bands from lane 1 and 2 (pDB.His.MBP&amp;nbsp; ?), purify and ligate together <br /> <br /> [[Image:0706 digestion.JPG]] <br /> <br /> Ligate and and transform, plate and let grow overnight <br /> <br /> === Colony Screening&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;June 9, 2012<br /> <br /> [[Image:0709_screening.JPG]]<br /> <br /> Digested MiniPrep plasmids with restriction enzymes Nhel and Xhol, positive inserts for samples 1, 2, 3 and 4<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> [[Category:Cloning]]</div>

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