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1247-pCDNA3.1-PBasic-PH - Revision history
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Xiaojing.Wang at 18:46, 19 July 2012
2012-07-19T18:46:03Z
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<p><b>New page</b></p><div>Cloning of pCDNA3.1-PBasic-PH(1247)<br>Purpose: to clone pCDNA3.1-PBasic-PH<br>Date: 2012-7-13 to 2012-7-19<br>Notebook: Xiaojing Wang<br>Page: 005296 <br />
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Clone ID: 1247<br>Experimenters: Xiaojing Wang <br />
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Results<br> <br />
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synthesize pBasic + PH<br> <br />
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[[Image:1247 PCR 7.16.2012.JPG|96x283px|1247 PCR 7.16.2012.JPG]]<br> <br />
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Digest with Xho1 + BamH1, ligate to Xho1 + BamH1 digested pCDNA3.1, 1226 <br> <br />
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[[Image:1247 i v digest 7.16.2012.JPG]]<br> <br />
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Transform DH5alpha <br />
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Pick up 6 clones to mini-prep<br>1247 plasmids are digested by Xho1 + BamH1<br> <br />
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[[Image:1247 digest 7.19.2012.JPG]]<br> <br />
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Clone 1247-1,-2,-3,-4,-5,-6 may be correct. <br />
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Pick up 1247-2 to sequence.<br> <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang