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1249 pET-Taq d289 - Revision history
2026-10-18T02:17:27Z
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Jennifer.truong at 16:28, 27 June 2012
2012-06-27T16:28:41Z
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<p><b>New page</b></p><div>== '''Cloning of 1249 pET-Taq d289''' ==<br />
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'''Purpose:''' Cloning of pET-Taq d289<br />
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'''Date: '''June 22, 2012 <br />
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'''Notebook:''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1249 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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Date: June 22, 2012 <br />
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[[Image:0622 Amp.JPG]] <br />
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No band in lane 3, expected size ~1.7 kb. Need to reamplify on Monday. <br> <br />
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Date:&nbsp;June 25. 2012 <br />
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[[Image:0625 Digestion.JPG]] <br />
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Cut out top band in lane 2, purified and digested <br />
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[[Image:0625_Digestion_2.JPG]]<br><br />
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Kpn1 + Nco1 digestion of the vector will cut out MBP, so cut the vector (the largest fragment), see circled fragment in lane 3. Cut out insert and vector, purified and ligated for an hour for transformation <br><br />
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=== Colony Screening ===<br />
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[[Image:0626 Screening.JPG]] <br />
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Grew samples 1, 3, 5, 6, 7 and 8 in media (lanes 9, 11, 13, 14 and 16) overnight <br />
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Date: June 27, 2012<br />
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[[Image:0627_Screening.JPG]]<br />
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Digested miniprep with enzymes to check if insert is still present<br />
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Samples 1, 2, 3 and 6 were positive (expected size:&nbsp;~1.7 kb)<br />
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Submitted sample 3 (lane 9)&nbsp;for sequencinng.<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Jennifer.truong