https://eln.excellgen.com/index.php?action=history&feed=atom&title=1268_pTXB1-RNaseA 2026-10-18T11:23:52Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1268_pTXB1-RNaseA&diff=5354&oldid=prev 2012-07-30T18:25:54Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1268 pTXB1-RNaseA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1268 pTXB1-RNaseA by gene synthesis &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;July 26, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; 0 <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1268 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Jennifer Truong&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;July 26, 2012&lt;br&gt; <br /> <br /> [[Image:0726 Amp.JPG]] <br /> <br /> See lane 1, amplified DNA sequence by gene synthesis (25 cycles, HotStartPhusion), expected size ~400 bp <br /> <br /> Cut out band, purify and digest with 1254 vector with RE Nde1 and Xho1 <br /> <br /> [[Image:1268_digestion.JPG]]<br /> <br /> See lane 1 (insert)&amp;nbsp;and lane 2 (vector), cut out purify and ligate together. Transform<br /> <br /> === Colony Screening ===<br /> <br /> Date: July 30, 2012<br /> <br /> [[Image:1268_1270_Screening.JPG]]<br /> <br /> Digested MiniPrep with RE Nde1 and Xho1<br /> <br /> Expected size ~400 bp<br /> <br /> Samples 3 and 5 (lanes 3 and 5 respectively) may be positive for insert<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> [[Category:Cloning]]</div>

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