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1270 pTXB1-RNaseT1 - Revision history
2026-10-18T08:19:20Z
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Xiaojing.Wang at 18:36, 30 July 2012
2012-07-30T18:36:41Z
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<p><b>New page</b></p><div>== '''Cloning of 1270 pTXB1-RNaseT1''' ==<br />
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'''Purpose:''' Cloning of 1270 pTXB1-RNaseT1 by gene synthesis <br> <br />
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'''Date: '''July 26, 2012<br> <br />
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'''Notebook:''' 0 <br />
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'''Page:''' <br />
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'''Clone ID: '''1270 <br />
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'''Experimenters:''' Jennifer Truong<br> <br />
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== '''Results''' ==<br />
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=== Cloning<br> ===<br />
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Date:&nbsp;July 26, 2012<br> <br />
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[[Image:0726 Amp.JPG]] <br />
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Amplified DNA sequence by gene synthesis, expected size ~400 bp (see lane 2) <br />
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Cut out band and purified <br />
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Digested with vector 1254 with RE Xho1 and Nde1 <br />
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[[Image:1270 digestion.JPG]] <br />
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See lanes 3 (insert)&nbsp;and 4 (vector) Cut out, purify and ligate together. <br />
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Transform <br />
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=== Colony Screening ===<br />
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Date:&nbsp;July 30, 2012<br />
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[[Image:1268_1270_Screening.JPG]]<br />
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Digested MiniPrep with Nde1 and Xho1 RE<br>Expected size 400 bp<br />
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Samples 4 (lane 8), 4 (lane 10)&nbsp;and 6 (lane 12) may have positive inserts <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion'''<br> ==<br />
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[[Category:Cloning]]</div>
Xiaojing.Wang