https://eln.excellgen.com/index.php?action=history&feed=atom&title=1274-pT7A3-RnaseH2 2026-10-18T09:24:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1274-pT7A3-RnaseH2&diff=12324&oldid=prev 2014-07-10T18:53:50Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1274-pT7A3-RnaseH2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1274-pT7A3-RnaseH2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-2 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1274&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Cloning Method <br /> <br /> PCR using genomic DNA from Escherichia coli str. K-12 substr. MG1655&lt;br&gt;RnaseH2-Nde1-F AGCTCTCATatgatcgaatttgtttatccgcac&lt;br&gt;RnaseH2-Xho1-R gtctcgctcgagggacgcaagtcccagtgcgcgt&lt;br&gt;clone into Nde1 + Xho1 digested 1892-pT7A3E&lt;br&gt; <br /> <br /> [[Image:1274 2210 2248 2197 PCR.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === 1274 DIGESTED ===<br /> <br /> [[Image:1274 2238 digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-7-01 <br /> <br /> Make miniprep and digest with Nde1 + XhoI <br /> <br /> [[Image:1274 1910 1535 2212 2230 Screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE4 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

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