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1274 pTXB1-RnaseH2 - Revision history
2026-10-18T04:02:39Z
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Xiaojing.Wang at 19:51, 6 August 2012
2012-08-06T19:51:33Z
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<p><b>New page</b></p><div>== '''Cloning of 1274 pTXB1-RnaseH2''' ==<br />
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'''Purpose:''' Cloning of 1274 pTXB1-RnaseH2 <br />
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'''Date: '''July 30, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1274 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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Date:&nbsp;July 30, 2012<br> <br />
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[[Image:0730 Amp.JPG]]<br> <br />
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See lane 2<br> <br />
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PCR using genomic DNA from Escherichia coli str. K-12 substr. MG1655<br>RnaseH2-Nde1-F AGCTCTCATatgatcgaatttgtttatccgcac<br>RnaseH2-Xho1-R gtctcgctcgagggacgcaagtcccagtgcgcgt<br>clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK <br />
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Expected size 615 bp<br> <br />
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Cut out band and save in box <br />
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Date: August 1, 2012<br> <br />
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[[Image:0801 Digestion.JPG]]<br> <br />
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Digested insert (lane 2)&nbsp;with RE Nde1 + Xho1, vector is not good, digest again tomorrow, join with lane 2, purify and ligate together <br> <br />
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=== Colony Screening ===<br />
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Date:&nbsp;August 6, 2012<br />
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Digested MiniPrep with RE Nde1 and Xho1, expected size 615 bp<br />
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[[Image:1274_0806_Screening.JPG]]<br />
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Bad digestion gel, digest again tomorrow<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Xiaojing.Wang