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1275 pTXB1-RNaseT - Revision history
2026-10-18T03:06:02Z
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Xiaojing.Wang at 19:55, 6 August 2012
2012-08-06T19:55:40Z
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<p><b>New page</b></p><div>== '''Cloning of 1275 pTXB1-RNaseT''' ==<br />
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'''Purpose:''' Cloning of 1275 pTXB1-RNaseT<br />
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'''Date: '''July 30, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1275 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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Date:&nbsp;July 30, 2012<br> <br />
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[[Image:0730 Amp.JPG]]<br> <br />
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See lane 3<br> <br />
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PCR using genomic DNA from K-12 substr. MG1655 <br />
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RnaseT-Nde1-F AGCTCTCATatgtccgataacgctcaacttac<br>RnaseT-Xho1-R gtctcgctcgagcacctcttcggcggcagatag<br>clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK<br> <br />
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Expected size 666 bp<br> <br />
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Cut out band and save in box<br />
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Date: August 1, 2012<br><br />
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[[Image:0801_Digestion.JPG]]<br><br />
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Digested insert (lane 3)&nbsp;with RE Nde1 + Xho1, vector is not good, digest again tomorrow, join with lane 3, purify and ligate together <br><br />
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=== Colony Screening ===<br />
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Date:&nbsp;August 6, 2012 <br />
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Digested MiniPrep with RE&nbsp;Xho1 and Nde1, expected size 666 bp <br />
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[[Image:1275 0806 Screening.JPG]] <br />
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Samples 1, 3, 4 5(?)&nbsp;may be correct <br />
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Send sample 3 to sequencing<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Xiaojing.Wang