Paul.xu at 19:36, 15 March 2013

2013-03-15T19:36:40Z

New page

== '''Cloning of 1278-pT7A3-S1''' ==

'''Purpose:''' Cloning of1278-pT7A3-S1

'''Date: '''2013-3-

'''Notebook:'''

'''Page:'''

'''Clone ID:1278'''

'''Experimenters:''' Shanyi

== '''Results''' ==

=== cloning ===

Date:2013-3- 

Gene synthesis of two fragments and join them by PCR digest with Nde1 + Xho1, clone into Nde1 + Xho1 digested  1279 pT7A3 

[[Image:1278-1pcr.JPG‎]][[Image:1278-2pcr.JPG‎ ]] 

then use Fragment1,2, as template

[[Image:1146_1278_1285pcr.JPG‎]] 

digest with Nde1+ Xho1

[[Image:Digest_for_1144_1592_1278.JPG‎]] 

 

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=== Colony Screening ===

Date: 2013

 

 

 

 

== '''Result intepretation/Summary''' ==

* 

== '''Conclusion''' ==

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[[Category:Cloning]]

1278-pT7A3-S1 - Revision history

Paul.xu at 19:36, 15 March 2013