https://eln.excellgen.com/index.php?action=history&feed=atom&title=1285_pT7A3-Proteinase-K 2026-10-18T08:19:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1285_pT7A3-Proteinase-K&diff=8719&oldid=prev 2013-04-01T13:48:33Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1285 pT7A3-Proteinase-K&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1285 pT7A3-Proteinase-K <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-3- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1285&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2013-3-&lt;br&gt; <br /> <br /> Gene synthesis of two fragments and join them by PCR&lt;br&gt;digest with Nde1 + Xho1, clone into Nde1 + Xho1 digested&amp;nbsp; 1279 pT7A3&lt;br&gt; <br /> <br /> [[Image:1285-1 1285-2pcr.JPG]]&lt;br&gt; <br /> <br /> then use Fragment1,2, as template <br /> <br /> [[Image:1146 1278 1285pcr.JPG]] <br /> <br /> digest with&amp;nbsp;Nde1+ Xho1 <br /> <br /> [[Image:Digest for1285 1535 1654.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013 <br /> <br /> [[Image:1285digest.JPG]][[Image:1285-236digest.JPG]]&lt;br&gt; <br /> <br /> [[Image:1285 2nddigest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu