https://eln.excellgen.com/index.php?action=history&feed=atom&title=1287-pFastbac1-VVDP 2026-10-18T06:19:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1287-pFastbac1-VVDP&diff=6266&oldid=prev 2012-09-28T14:07:11Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1287-pFastbac1-VVDP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1287-pFastbac1-VVDP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-8-21---2012-8-24 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1287&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> template: vaccinia virus genomic DNA <br /> <br /> VVDP-BamH1-6H-F &lt;br&gt; <br /> <br /> VVDP-Xho1-R &lt;br&gt; <br /> <br /> [[Image:1287+1324 PCR 8-21-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest PCR product with BamH1 + Xho1, clone into BamH1 + Xho1, 292 pFastBac1&lt;br&gt; <br /> <br /> [[Image:1287+1346 clone 8-22-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-08-24 <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1287 Screening.JPG]] <br /> <br /> Digested MiniPrep with RE Nde1 <br /> <br /> [[Image:1287 Digestion 2.JPG]] <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of 1287-pFastbac1-VVDP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1287-pFastbac1-VVDP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-8-29---2012-8-31 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1287&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> template: vaccinia virus genomic DNA <br /> <br /> VVDP-BamH1-6H-F &lt;br&gt; <br /> <br /> VVDP-Xho1-R &lt;br&gt; <br /> <br /> [[Image:1287+1324 PCR 8-21-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest PCR product with BamH1 + Xho1, clone into BamH1 + Xho1, 292 pFastBac1&lt;br&gt; <br /> <br /> [[Image:1272+1287_clone_8-29-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-08-31 <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1287+1346+1361digest_detect_8-31-2012.JPG]]&lt;br&gt; <br /> <br /> no insert<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

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