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1288-pCMV52-Fc(3c)-NPR1-ECD - Revision history
2026-10-18T14:42:46Z
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Paul.xu at 16:07, 23 August 2012
2012-08-23T16:07:25Z
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<p><b>New page</b></p><div>= == '''Cloning of 1288-pCMV52-Fc(3c)-NPR1-ECD'''<br> =<br />
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'''Purpose:''' Cloning of 1288-pCMV52-Fc(3c)-NPR1-ECD <br />
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'''Date: '''2012-8-21---2012-8-23 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1288''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1288 PCR 8-6-2012.JPG]]<br> <br> <br />
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[[Image:1288+1224 clone 8-21-2012.JPG]]<br> <br />
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band 2:digest 1276 pCMV52-Fc(3c)-NPR1-ECD with Xho1 + BamH1<br>band 1:digest PCR product with Sal1 + BamH1.<br> <br />
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=== Colony Screening ===<br />
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Date: 2012-8-23 <br />
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Make miniprep and digest with Xho1 + BamH1,Nde1 + Bamh1<br> <br />
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[[Image:1288_digest_detect_8-23-2012.JPG]]<br><br />
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clone 1,2,3,4,5,6 may be right<br />
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== '''Result intepretation/Summary''' ==<br />
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*&amp;amp;lt;br&amp;amp;gt;<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu