https://eln.excellgen.com/index.php?action=history&feed=atom&title=1291_pET30b-TAT-NLS-sso7d 2026-10-18T02:19:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1291_pET30b-TAT-NLS-sso7d&diff=5508&oldid=prev 2012-08-09T18:49:59Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1291 pET30b-TAT-NLS-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1291 pET30b-TAT-NLS-sso7d <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;July 31, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1291 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date: July 31, 2012&lt;br&gt; <br /> <br /> [[Image:1291 Amp.JPG]]&lt;br&gt; <br /> <br /> Gene synthesis of PCR&amp;nbsp;product. Expected size ~100 bp&lt;br&gt; <br /> <br /> See lane 2&lt;br&gt; <br /> <br /> Cut out and purify band, digest with RE&amp;nbsp; Nde1 + Kpn1&amp;nbsp; (lane 1) with vector 368 pET30b-sso7d-KX (lane 2)&lt;br&gt; <br /> <br /> [[Image:1291 Digestion.JPG]] <br /> <br /> Cut out bands, purify and ligate over night <br /> <br /> Date:&amp;nbsp;August 6, 2012&lt;br&gt;<br /> <br /> MiniPrep screening came back all negative, started all over again&lt;br&gt;<br /> <br /> [[Image:1291_0806_Amp.JPG]]&lt;br&gt;<br /> <br /> Gene synthesis of PCR product. Expected size ~100 bp<br /> <br /> See lane 2<br /> <br /> Cut out and purify band, digest with RE Nde1 + Kpn1 (lane 1) with vector 368 pET30b-sso7d-KX (lane 2)&lt;br&gt;<br /> <br /> [[Image:1291_0806_Digestion.JPG]]&lt;br&gt;<br /> <br /> Cut out bands, purify and ligate over night &lt;br&gt;<br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> [[Image:1291 Screening.JPG]]&lt;br&gt; <br /> <br /> Digested MiniPrep with RE Nde1 + Kpn1, no inserts&lt;br&gt; <br /> <br /> Date:&amp;nbsp;August 9, 2012&lt;br&gt; <br /> <br /> [[Image:1291_0809_Screening.JPG]]<br /> <br /> Digested MIniPrep with RE&amp;nbsp;Nde1 and Kpn1, faint inserts at expected ~100 bp<br /> <br /> 1, 2, 3, 5 and 6 may be correct<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

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