https://eln.excellgen.com/index.php?action=history&feed=atom&title=1293pET30b-2Tat-6Arg 2026-10-18T08:19:13Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1293pET30b-2Tat-6Arg&diff=6998&oldid=prev 2012-11-14T17:39:48Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1293pET30b-2Tat-6Arg&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1293pET30b-2Tat-6Arg <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Nov 1, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1293 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;Nov 1, 2012 <br /> <br /> &lt;br&gt; <br /> <br /> Synthesized template via gene synthesize, expected size ~147 bp <br /> <br /> &lt;br&gt; <br /> <br /> Digested insert&amp;nbsp; and&amp;nbsp; vector&amp;nbsp; with&amp;nbsp; Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1290V15 1292368 1293V15 1352 13041280DIGEST 10 26 2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Ligate , transform&amp;nbsp; <br /> <br /> [[Image:1293digest11_07_2012.JPG]][[Image:1293-3digest11_07_2012.JPG]]<br /> <br /> digest with NdeI and XhoI&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu