https://eln.excellgen.com/index.php?action=history&feed=atom&title=1299_pET-6H-VPMLK-NLS 1299 pET-6H-VPMLK-NLS - Revision history 2026-10-18T04:26:01Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1299_pET-6H-VPMLK-NLS&diff=5616&oldid=prev Jennifer.truong at 19:57, 15 August 2012 2012-08-15T19:57:14Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1299 pET-6H-VPMLK-NLS&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1299 pET-6H-VPMLK-NLS<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;August 13, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1299 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;August 13, 2012 <br /> <br /> Ligate annealed oligos to Nco1 + Nde1 digested 1128 pDB.His.PfuMBP-(tev) vector, see lane 1&lt;br&gt;His-VPMLK-NLS-T&lt;br&gt;cATGGCACATCATCATCACCATCATGTTCCGATGCTGAAACCGAAAAAAAAACGCAAAGTGca&lt;br&gt; cGTGTAGTAGTAGTGGTAGTACAAGGCTACGACTTTGGCTTTTTTTTTGCGTTTCACgtat&lt;br&gt;His-VPMLK-NLS-B &lt;br&gt;tatgCACTTTGCGTTTTTTTTTCGGTTTCAGCATCGGAACATGATGGTGATGATGATGTG&lt;br&gt; <br /> <br /> [[Image:0813 Vector Digestion.JPG]] <br /> <br /> Transform <br /> <br /> Date: August 15, 2012 <br /> <br /> Ligate annealed oligos to Nco1 + Nde1 digested pDB.His.MBP, V14 vector, see lane 1&lt;br&gt;His-VPMLK-NLS-T&lt;br&gt;cATGGCACATCATCATCACCATCATGTTCCGATGCTGAAACCGAAAAAAAAACGCAAAGTGca&lt;br&gt;cGTGTAGTAGTAGTGGTAGTACAAGGCTACGACTTTGGCTTTTTTTTTGCGTTTCACgtat&lt;br&gt;His-VPMLK-NLS-B&lt;br&gt;tatgCACTTTGCGTTTTTTTTTCGGTTTCAGCATCGGAACATGATGGTGATGATGATGTG&lt;br&gt; <br /> <br /> [[Image:0815 Vector Digestion.JPG]] <br /> <br /> Transform<br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Jennifer.truong