https://eln.excellgen.com/index.php?action=history&feed=atom&title=129_pL42-TthMuts 2026-10-18T05:48:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=129_pL42-TthMuts&diff=19053&oldid=prev 2018-08-17T21:45:18Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 129 pT7A30-TthMuts&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 129 pL42-TthMuts<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;06, 27, 2018<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 129&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR from 212 pL6H-TthMuts<br /> <br /> &#039;&#039;&#039;PCR of 129&#039;&#039;&#039;<br /> <br /> [[File:IMG 1027.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;redo PCR&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Template:212&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;commerical 2xPCR mix,5x enhancer&#039;&#039;&#039;<br /> <br /> [[File:IMG 1118.JPG|RTENOTITLE]]<br /> <br /> == Date:8/4/2018 ==<br /> <br /> &#039;&#039;&#039;redo PCR&#039;&#039;&#039;<br /> <br /> [[File:IMG 1202.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> Vector is 2289<br /> <br /> [[File:IMG 1263.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;Miniprep of 129_994_1000_3206&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Vector<br /> | Lane<br /> | Enzymes<br /> | Size<br /> | Clone#<br /> | Note<br /> |-<br /> | 129<br /> | 1801<br /> | 1-4<br /> | Nde1+HindIII<br /> | 2433<br /> | 2,4 look good<br /> | &#039;&#039;&#039;129-2;129-4&#039;&#039;&#039;<br /> |-<br /> | 994<br /> | 3225<br /> | 5-8<br /> | BamH1+Xho1<br /> | 609<br /> | 5,6,look good<br /> | Vecotor doesn&#039;t have BamH1 Site,&#039;&#039;&#039;994-1;994-2&#039;&#039;&#039;<br /> |-<br /> | 1000<br /> | 1708<br /> | 9-12<br /> | HIndIII+Xho1<br /> | 118<br /> | &lt;br/&gt;<br /> | 11-13 good<br /> |-<br /> | 3206<br /> | 3165<br /> | 13-16<br /> | HIndIII+BamH1<br /> | 1460<br /> | &lt;br/&gt;<br /> | need to do PCR screening<br /> |}<br /> <br /> [[File:IMG 1053.JPG|RTENOTITLE]]<br /> <br /> wrong vector<br /> <br /> Date:7/26/2018<br /> <br /> &#039;&#039;&#039;129 PCR screening&#039;&#039;&#039;<br /> <br /> [[File:IMG 1214.JPG|RTENOTITLE]]<br /> <br /> == Date:8/7/2018 ==<br /> <br /> &#039;&#039;&#039;129 PCR Screening&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Primers:T7-R+212-F&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Result:#1-7 are good, grow up in kana&#039;&#039;&#039;<br /> <br /> [[File:IMG 1278.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 129&#039;&#039;&#039;<br /> <br /> [[File:IMG 1279.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div>

Crm