https://eln.excellgen.com/index.php?action=history&feed=atom&title=1303_pTXB1-Cre 2026-10-18T10:35:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1303_pTXB1-Cre&diff=5827&oldid=prev 2012-08-27T18:00:06Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1303 pTXB1-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1303 pTXB1-Cre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;August 24, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1303 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;August 24, 2012 <br /> <br /> See lane 5 <br /> <br /> [[Image:1303 Amp.JPG]]&lt;br&gt; <br /> <br /> PCR using 298 pTriEx-HTNC as template&lt;br&gt;Cre-Nde1-F&lt;br&gt;cagtca catatg tcc aat tta ctg acc gta cac&lt;br&gt;Cre-Xho1-R&lt;br&gt;(gaagatggcgattagctcgag ctgaca)&lt;br&gt;tgtcagctcgagctaatcgccatcttc&lt;br&gt;clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK&lt;br&gt; <br /> <br /> [[Image:1303 Digestion.JPG]]&lt;br&gt; <br /> <br /> Digested DNA (lane 5)&amp;nbsp;and vector 1254 pTXB1-PT-ELK with RE Nde1 + Xho1&lt;br&gt; <br /> <br /> Cut out and purify, ligate together, transform <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> 2012-8-27&lt;br&gt;<br /> <br /> Nde1 + Xho1&lt;br&gt;<br /> <br /> [[Image:1303+1335+1353_digest_detct_8-27-2012.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu