https://eln.excellgen.com/index.php?action=history&feed=atom&title=1307-1309-1310_screening 1307-1309-1310 screening - Revision history 2026-10-18T19:24:27Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1307-1309-1310_screening&diff=7304&oldid=prev Paul.xu at 23:20, 3 December 2012 2012-12-03T23:20:25Z <p></p> <p><b>New page</b></p><div>Data:2012-10-30--2012-11-8 <br /> <br /> Exprimenter: Xiuli <br /> <br /> 2 ml sample each , 1307, 1308, 1309&amp;nbsp; induce with 0.5mM IPTG at&amp;nbsp; 37 oC for 4-5 hours&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 95px;&quot;<br /> |-<br /> | &#039;&#039;&#039;1307&#039;&#039;&#039;<br /> | &#039;&#039;&#039;pET-6H-VPTLK-NLS-Cre&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1309&#039;&#039;&#039;<br /> | &#039;&#039;&#039;pET-6H-KLPVM-NLS-Cre&#039;&#039;&#039;<br /> |-<br /> | &#039;&#039;&#039;1310&#039;&#039;&#039;<br /> |&#039;&#039;&#039;pET-6H-VPMLK-NLS-Cre&#039;&#039;&#039;<br /> |}<br /> &lt;br&gt;<br /> <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane <br /> | 1 <br /> | 2 <br /> | 3 <br /> | 4 <br /> | 5 <br /> | 6 <br /> | 7 <br /> | 8 <br /> | 9 <br /> | 10 <br /> | 11 <br /> | 12<br /> |-<br /> | IPTG <br /> | 0 <br /> | 0.5 <br /> | 0 <br /> | 0.5 <br /> | 0 <br /> | 0.5 <br /> | 0 <br /> | 0.5 <br /> | 0 <br /> | 0.5 <br /> | 0 <br /> | 0.5<br /> |-<br /> | lane <br /> | Sup <br /> | Sup <br /> | Pellet <br /> | Pellet <br /> | Sup <br /> | Sup <br /> | Pellet <br /> | Pellet <br /> | Sup <br /> | Sup <br /> | Pellet <br /> | Pellet<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1307-09-1310 screening on 11-08-2012.jpg]] [[Image:1310 pellet.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;413&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu