https://eln.excellgen.com/index.php?action=history&feed=atom&title=1315-pT7A3-MthRnl 2026-10-18T03:29:18Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1315-pT7A3-MthRnl&diff=10881&oldid=prev 2014-01-06T15:19:09Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1315-pT7A3-MthRnl&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1315-pT7A3-MthRnl <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-11-3---2013-11-6 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1315&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1315 1891pcr 10-30-2013.JPG]]&lt;br&gt; <br /> <br /> pcr1:MthRnl-1-1 to MthRnl-1-16&lt;br&gt; <br /> <br /> pcr2:MthRnl-2-1 to MthRnl-2-16&lt;br&gt; <br /> <br /> [[Image:1315 1891PCR 10-30-2013.JPG]]&lt;br&gt; <br /> <br /> PCR:MthRnl-1-1 +MthRnl-2-16&lt;br&gt; <br /> <br /> [[Image:1315clone 11-4-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1225,pET28b-Endo1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-11-6 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1315digest 11-6-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1315-pT7A3-MthRnl&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1315-pT7A3-MthRnl <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-12-30---2014-1-2 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1315&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> [[Image:1146 1315 1946pcr 12-30-2013.JPG]]&lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt; <br /> <br /> pcr1:MthRnl-1-1&amp;nbsp; to MthRnl-1-16&lt;br&gt; <br /> <br /> pcr2:MthRnl-2-1&amp;nbsp; to MthRnl-2-16&lt;br&gt; <br /> <br /> [[Image:1315 1935 1943 1946 1984PCR 12-31-2013.JPG]]&lt;br&gt; <br /> <br /> PCR:MthRnl-1-1+ MthRnl-2-16&lt;br&gt; <br /> <br /> [[Image:1315 1943 1946 1984 1991clone 12-31-2013.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR &lt;br&gt; <br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-2 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 &lt;br&gt; <br /> <br /> [[Image:1315 1943digest 1-2-2014.JPG]]&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu