https://eln.excellgen.com/index.php?action=history&feed=atom&title=1319A_pEC2-T4-BGT 1319A pEC2-T4-BGT - Revision history 2026-10-18T02:31:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1319A_pEC2-T4-BGT&diff=13381&oldid=prev Excellgen at 20:28, 10 October 2014 2014-10-10T20:28:32Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1319A pEC2-T4-BGT&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1319 pEC2-T4-BGT&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1319&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> pGene synthesis Fragment 1,2 <br /> <br /> [[Image:2297 1038 1319PCR.JPG]] <br /> <br /> join two fragments by PCR <br /> <br /> [[Image:1319pcr.JPG]] <br /> <br /> digest with Nde1 + XhoI&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2297 1504 1319 2240 2241 2246 2199.JPG]] <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:1319A 1504A 2199A 2240A 2241A 2297DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === fix 1319-3pT7A3E-T4-BGT ===<br /> <br /> pcr using&lt;br&gt;<br /> <br /> T4-BGT-1-1+T4-BGT-fix-1+T4-BGT-1-2+T4-BGT-2-16&lt;br&gt;<br /> <br /> [[Image:2321_1319fix_1916fixpcr.JPG]]&lt;br&gt;<br /> <br /> digest with Nde1 + XhoI&lt;br&gt; <br /> <br /> [[Image:Digest_for_1319fix_1916fix_2360_2361_2321.JPG]]&lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:1319FIX_1916FIX_2360_2361_2321DIGEST.JPG]]&lt;br&gt; <br /> <br /> use T7 term primer+T4-BGT-2-16 to do PCR&lt;br&gt;<br /> <br /> [[Image:1319FIX_1916FIX_pcrSCREENING.JPG]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen