https://eln.excellgen.com/index.php?action=history&feed=atom&title=1322-pT7A3-mutY 1322-pT7A3-mutY - Revision history 2026-10-18T03:12:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1322-pT7A3-mutY&diff=11100&oldid=prev Paul.xu: Created page with "== '''Cloning of 1322-pT7A3-mutY''' == '''Purpose:''' Cloning of 1322-pT7A3-mutY '''Date: '''2014-1-15---2014-1-23 '''Notebook:0''' '''Page:''' '''Clone ID:1322''' '''E..." 2014-01-28T15:55:00Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1322-pT7A3-mutY&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1322-pT7A3-mutY &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-15---2014-1-23 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1322&#039;&#039;&#039; &#039;&#039;&#039;E...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1322-pT7A3-mutY&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1322-pT7A3-mutY<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1-15---2014-1-23<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1322&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715_718_1114_1142_1322_1972_2015PCR_1-15-2014.jpg]]&lt;br&gt;<br /> <br /> PCR from E coli genomic DNA&lt;br&gt;MutY-Nde1-F&lt;br&gt;MutY-Xho1-R&lt;br&gt;<br /> <br /> [[Image:1322_1972clone_1-15-2014.jpg]]<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-1-23<br /> <br /> Make miniprep and digest with Nde1 +Xho1&lt;br&gt;<br /> <br /> [[Image:1972_1322_2024digest_1-23-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu