Paul.xu at 13:18, 9 October 2012

2012-10-09T13:18:58Z

New page

== '''Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT''' ==

'''Purpose:''' Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT

'''Date: '''2012-8-14---2012-8-15

'''Notebook:0'''

'''Page:'''

'''Clone ID:1324'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:1305+1324 PCR 8-14-2012.JPG]] 

 

[[Image:1271+1305+1324 clone 8-14-2012.JPG]]

{| width="426" cellspacing="1" cellpadding="1" border="1"
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| lane 5 
| lane 6 
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| PCR product 
| 1224 pCMV5-Fc(3C)-Dest-SVLT 
|-
| enzymes 
| colspan="2" |      Nhe1 + Not1 
|}

'''1224 vector should not have three lanes''' 

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=== Colony Screening ===

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

&amp;lt;br&amp;gt;

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&amp;lt;br&amp;gt;

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&lt;br&gt;

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== '''Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT''' ==

'''Purpose:''' Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT

'''Date: '''2012-8-30---2012-9-4

'''Notebook:0'''

'''Page:'''

'''Clone ID:1324'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:1287+1324 PCR 8-21-2012.JPG]] 

 

[[Image:1298+1324+1346+1347+1350+1356 clone 8-31-2012.JPG]]

 

 

 

{| width="426" cellspacing="1" cellpadding="1" border="1"
|-
| 
| lane 9 
| lane 10 
|-
| 
| PCR product 
| 1224 pCMV5-Fc(3C)-Dest-SVLT 
|-
| enzymes 
| colspan="2" |      Nhe1 + Not1 
|}

 

{| cellspacing="1" cellpadding="1" border="1"
|-
| 
|}

=== Colony Screening ===

Date: 2012-9-4

Make miniprep and digest with Nhe1 + Not1 

[[Image:1324 digest detct 9-4-2012.JPG]]

 

not the right size

 

9/8/2012, digest miniprep with Nhe1 + Not1. no insert. 

[[Image:1324 9 8 2012.JPG]]

 

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

&amp;lt;br&amp;gt;

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== '''Cloning of  pCMV5-Fc(3C)-VVDP-WPRE-OriP''' ==

'''Purpose:''' Cloning of pCMV5-Fc(3C)-VVDP-WPRE-OriP

'''Date:'''  2012-9-28 ~2012-10-5

'''Notebook:0'''

'''Page:'''

'''Clone ID: '''

'''Experimenters:''' Yingying

== '''Results''' ==

=== PCR ===

2012-9-28 

[[Image:1287+1324 PCR 9-28-2012.JPG]] 

 

[[Image:1287+1324 clone 10-1-2012.JPG]]

digest PCR product( lane3) with Nhe1+Not1

digest 1349pCMV5-Fc-(3c)-Dest-WPRE-oriP with  Nhe1+Not1

 

'''Colony Screening'''

Date: 2012-10-5 

mini prep and digest with Nhe1+Not1 

[[Image:1413+1414+1324_digest_detect_10-5-2012.JPG]]

clone 5 is right

 

== '''Result intepretation/Summary''' ==

 

== '''Conclusion''' ==

[[Category:Cloning]]

1324-pCMV5-Fc(3C)-VVDP-SVLT - Revision history

Paul.xu at 13:18, 9 October 2012