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1328 pCMV5-Fc(3C)-Dest - Revision history
2026-10-18T03:29:23Z
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Paul.xu at 21:47, 17 August 2012
2012-08-17T21:47:07Z
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<p><b>New page</b></p><div>== '''Cloning of 1328_pCMV5-Fc(3C)-Dest''' ==<br />
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'''Purpose:''' Cloning of&nbsp;1328_pCMV5-Fc(3C)-Dest <br />
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'''Date: '''2012-8-10 to 2012-8-13<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: 1328''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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Date:2012-2-10 8/10/2012 <br />
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digest pCMV5-Fc(3C) 1262 with Nhe1 + Not1 --&gt; vector<br>digest 1224 pCMV5-Fc(3C)-Dest-SVLT with Nhe1 + Not1 --&gt; gateway cassette, truncated<br>ligate and transform ccdb-survial<br> <br />
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[[Image:1328 8 10 2012.jpg]]<br> <br />
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=== Colony Screening ===<br />
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Date:2012-8-13 <br />
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Make miniprep and digest with Nhe1 + Not1 <br />
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[[Image:1328 digest detect 8-13-2012.JPG]] <br />
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all right <br />
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Gateway reaction<br> <br />
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2012-8-15----2012-8-16<br> <br />
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clony screening: digest with Nhe1 + Not1 <br> <br />
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[[Image:393+1328 8-16-2012.JPG]]<br> <br />
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clone 3,4 may be right; <br />
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7:control, 1328 plasmid <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu