https://eln.excellgen.com/index.php?action=history&feed=atom&title=1338_pDB.His.MBP-%283C%29-Amp 2026-10-18T09:49:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1338_pDB.His.MBP-(3C)-Amp&diff=7045&oldid=prev 2012-11-14T20:27:37Z

<p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1338 pDB.His.MBP-(3C)-Amp&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1338 pDB.His.MBP-(3C)-Amp &#039;&#039;&#039;Date: &#039;&#039;&#039;Nov 5, 2012&lt;br&gt; &#039;&#039;&#039;Notebook: &#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Cl...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1338 pDB.His.MBP-(3C)-Amp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1338 pDB.His.MBP-(3C)-Amp <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Nov 5, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1338 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date: Nov5, 2012&lt;br&gt; <br /> <br /> digest pET30b.Amp (V16) with Xba1 + Xho1--&amp;gt; vector&lt;br&gt;digest 1207 pDB.His.MBP-(3C) with Xba1 + Xho1--&amp;gt;insert<br /> <br /> ligate and transform, grow on LB/AMP plate,&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> <br /> <br /> [[Image:1304_1338digest_11_13_2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> Xba1 + Xho1&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu