https://eln.excellgen.com/index.php?action=history&feed=atom&title=1339_pDB.His.MBP-%283C%29.amp-Dest 2026-10-18T05:51:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1339_pDB.His.MBP-(3C).amp-Dest&diff=7071&oldid=prev 2012-11-16T18:20:24Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of == &#039;1339 pDB.His.MBP-(3C).amp-Dest&#039;&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1339 pDB.His.MBP-(3C).amp-Dest <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Nov 14, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1339 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date: Nov14, 2012&lt;br&gt; <br /> <br /> [[Image:1352 1298 1328 1338 1454fdigest11 14 2012.JPG]] <br /> <br /> digest 1338 pDB.His.MBP-(3C)-Amp with Nhe1 + Not1--&amp;gt; vector&lt;br&gt;digest 1328 pCMV5-Fc(3C)-Dest with Nhe1+ Not1--&amp;gt; gateway cassette, short version&lt;br&gt;ligate and transform ccdbSurvival, grow on LB/amp plate&lt;br&gt; <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> [[Image:1312_1339_1454digest11_16_2012.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; &#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu