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1342 pDB.His.MBP-(3C)-p53 (1-363) - Revision history
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Paul.xu at 16:58, 27 August 2012
2012-08-27T16:58:47Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1342 pDB.His.MBP-(3C)-p53 (1-363)''' ==<br />
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'''Purpose:''' Cloning of 1342 pDB.His.MBP-(3C)-p53 (1-363) <br />
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'''Date: '''August 24, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1342 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning<br> ===<br />
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Date:&nbsp;August 24, 2012 <br />
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PCR from 80 pLex-p53wt <br />
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P53-Nhe1-F<br>atcgac GCTAGC ATGGAGGAGCCGCAGTCAGATC <br />
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P53-EcoR1-R2<br>(AAGGAGCCAGGGGGGAGCAGGTGA gaattc atcgac)<br>gtcgatgaattcTCACCTGCTCCCCCCTGGCTCCTT<br> <br />
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See lane 2 <br />
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[[Image:1342 Amp.JPG]] <br />
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Digested insert (lane 4) and vector 1207 pDB.His.MBP-(3C) (lane 5)&nbsp;with RE Nhe1 and EcoR1<br> <br />
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[[Image:1343 Digestions.JPG]]<br> <br />
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Cut out bands, ligate together, transform <br />
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=== <u></u>Colony Screening<br> ===<br />
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2012-8-27<br><br />
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Nhe1 and EcoR1<br><br />
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[[Image:1342+1343+1344_digest_detect_8-27-2012.JPG]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu