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1344 pDB.His.MBP-(3C)-PLK1wt - Revision history
2026-10-18T11:23:03Z
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Paul.xu at 17:01, 27 August 2012
2012-08-27T17:01:01Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1344 pDB.His.MBP-(3C)-PLK1wt''' ==<br />
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'''Purpose:''' Cloning of 1344 pDB.His.MBP-(3C)-PLK1wt <br />
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'''Date: '''August 24, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1344 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning<br> ===<br />
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Date:&nbsp;August 24, 2012 <br />
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Cloning Method <br />
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PCR from 73 pLex-PLK1-wt<br>PLK1-Nhe1-F<br>cagtacGCTAGCATGAGTGCTGCAGTGACTGC<br>PLK1-EcoR1-R<br>(CAACCGTCTCAAGGCCTCCTAAgaattc ctagac)<br>gtctaggaattcTTAGGAGGCCTTGAGACGGTT<br><br> <br />
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See lane 4 <br />
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[[Image:1344 Amp.JPG]] <br />
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Digested DNA (see lane 4)&nbsp;with RE Nhe1 +&nbsp;EcoR1 <br />
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[[Image:0824 Digestion.JPG]] <br />
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Digested vector 1207 (see lane 3)&nbsp;with RE Nhe1 +&nbsp;EcoR1 <br />
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[[Image:Vector Digestions.JPG]] <br />
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Cut out bands, purify, ligate together, transform <br />
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=== <u></u>Colony Screening<br> ===<br />
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2012-8-27<br><br />
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Nhe1 and EcoR1<br><br />
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[[Image:1342+1343+1344_digest_detect_8-27-2012.JPG]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu