https://eln.excellgen.com/index.php?action=history&feed=atom&title=1347-pAd52-PR8-HAwt 2026-10-18T08:48:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1347-pAd52-PR8-HAwt&diff=5899&oldid=prev 2012-09-04T18:27:53Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1347-pAd52-PR8-HAwt&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1347-pAd52-PR8-HAwt <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-8-31----2012-9-4<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1347&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR using 57 pHW2000-PR8-HAwt as template and &lt;br&gt;PR8-HAwt-BamH1-F &lt;br&gt;PR8-HAwt-Not1-R&lt;br&gt; <br /> <br /> [[Image:1347PCR 8-31-2012.JPG]] <br /> <br /> [[Image:1298+1324+1346+1347+1350+1356 clone 8-31-2012.JPG]]&lt;br&gt; <br /> <br /> BamH1 + Not1 digested 1070 pAd52-CMV5-Cuo-IRES-EGFP as vector&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-9-4 <br /> <br /> Make miniprep and digest with BamH1 + Not1&lt;br&gt; <br /> <br /> [[Image:1346+1347+1350_digest_detct_9-4-2012.JPG]]<br /> <br /> clone 1,2,3,4,5 may be right<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu