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134 pL42-TthMuts - Revision history
2026-10-18T03:29:46Z
Revision history for this page on the wiki
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Crm at 17:16, 14 October 2018
2018-10-14T17:16:05Z
<p></p>
<p><b>New page</b></p><div>Subcloning:<br />
<br />
For 134: vector 212 and&nbsp; insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1<br />
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For 136: Vector 2038 and insert&nbsp; 129, Nco1 - Xho1&nbsp;<br />
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[[File:3257 212 1708 129 2038.JPG|RTENOTITLE]]<br />
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Miniprep digestion<br />
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[[File:134 136 135 3164 digestionJPG.jpeg|RTENOTITLE]]<br />
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Repeat<br />
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10/7/2018<br />
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Lane 1: 129: Nde`+ Not1<br />
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Lane 2: 2038: Nde`-Not1<br />
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[[File:129 2038.JPG|RTENOTITLE]]<br />
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http://www.excellgen.net/eln/images/3/37/1708_212_3257.JPG<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| Lane<br />
| ID<br />
| Name<br />
| Enzyme<br />
|-<br />
| 1<br />
| 129<br />
| <br/><br />
| Nde1-Not1<br />
|-<br />
| 2<br />
| 2038<br />
| <br/><br />
| Nde1-Not1<br/><br />
|-<br />
| 3<br />
| 1708<br />
| <br/><br />
| Nco1-Xho1<br />
|-<br />
| 4<br />
| 212<br />
| <br/><br />
| Nco1-Xho1<br/><br />
|-<br />
| 5<br />
| 3257<br />
| <br/><br />
| EcoR1-Nhe1<br />
|-<br />
| 6<br />
| 3260<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 7<br />
| 3261<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 8<br />
| 3259<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 9<br />
| 3258<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 10<br />
| 3262<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|}<br />
<br />
10/9/2018<br />
<br />
[[File:134 136 plasmid digestion.JPG|RTENOTITLE]]<br />
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10/10/2018[[File:129 2038 Nde Not.JPG|RTENOTITLE]]<br />
<br />
<br />
<br />
[[File:134 3274 129 2038 212.JPG|RTENOTITLE]]<br />
<br />
[[File:134 129 2038 3274 212 Not1-Nde1.JPG]]<br />
[[Category:Cloning]]</div>
Crm