https://eln.excellgen.com/index.php?action=history&feed=atom&title=1350-pTT5-MCS 1350-pTT5-MCS - Revision history 2026-10-18T07:06:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1350-pTT5-MCS&diff=5900&oldid=prev Paul.xu at 18:29, 4 September 2012 2012-09-04T18:29:41Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1350-pTT5-MCS&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1350-pTT5-MCS <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-8-30---2012-9-4<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1350&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR out CMV5 promoter tail using <br /> <br /> Pcmv5-Xho1-F&lt;br&gt;GATCGGAAAACCTCTCGAGAAAG <br /> <br /> CMV5-BglII-R &lt;br&gt; <br /> <br /> [[Image:1298+1348p1+1350 PCR 8-31-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1298+1324+1346+1347+1350+1356 clone 8-31-2012.JPG]]&lt;br&gt; <br /> <br /> digest vector 390 SI1-Unc5b with Xho1 + BamH1. ---&amp;gt; vector&lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-9-4<br /> <br /> Make miniprep and digest with Xho1 + BamH1&lt;br&gt; <br /> <br /> [[Image:1346+1347+1350_digest_detct_9-4-2012.JPG]]&lt;br&gt;<br /> <br /> <br /> <br /> Make miniprep and digest with Xho1 + Xba1&lt;br&gt; <br /> <br /> [[Image:1356+1358+1350_digest_detect_9-4-2012.JPG]]<br /> <br /> clone 1,3,4,5 may be right <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu