https://eln.excellgen.com/index.php?action=history&feed=atom&title=1352-pET28b-Cre 2026-10-18T05:13:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1352-pET28b-Cre&diff=6553&oldid=prev 2012-10-15T19:23:33Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1352-pET28b-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1352-pET28b-Cre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;August 30, 2012---2012-9-4&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1352 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yingying lu <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;August 30, 2012 <br /> <br /> [[Image:1331+1341+1352 PCR 8-30-2012.JPG]]&lt;br&gt; <br /> <br /> PCR using 298 pTriEx-HTNC as template&lt;br&gt;Cre-Nde1-F&lt;br&gt;cagtca catatg tcc aat tta ctg acc gta cac&lt;br&gt;Cre-Xho1-R&lt;br&gt;(gaagatggcgattagctcgag ctgaca)&lt;br&gt;tgtcagctcgagctaatcgccatcttc&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> 2012-8-31 <br /> <br /> no colonies <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1352-pET28b-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1352-pET28b-Cre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-10-10----2012-10-15&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1352 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yingying lu <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date: , 2012 <br /> <br /> [[Image:1331+1341+1352 PCR 8-30-2012.JPG]]&lt;br&gt; <br /> <br /> PCR using 298 pTriEx-HTNC as template&lt;br&gt;Cre-Nde1-F&lt;br&gt;cagtca catatg tcc aat tta ctg acc gta cac&lt;br&gt;Cre-Xho1-R&lt;br&gt;(gaagatggcgattagctcgag ctgaca)&lt;br&gt;tgtcagctcgagctaatcgccatcttc&lt;br&gt; <br /> <br /> [[Image:240+1097+1107+1352+1423_clone_10-12-2012.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;379&quot; height=&quot;100&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | lane 12&lt;br&gt;<br /> | lane 13&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1026- pET28b-LambdaExo&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> 2012-10-15 <br /> <br /> [[Image:1352+1423_digest_detect_10-15-2012.JPG]]&lt;br&gt;<br /> <br /> clone 2,3,4 need to sequence<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu