https://eln.excellgen.com/index.php?action=history&feed=atom&title=1353-1305-1369_transfection_on_0912-2012 1353-1305-1369 transfection on 0912-2012 - Revision history 2026-10-18T05:56:19Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1353-1305-1369_transfection_on_0912-2012&diff=6074&oldid=prev Xiuli.huang at 16:44, 18 September 2012 2012-09-18T16:44:55Z <p></p> <p><b>New page</b></p><div>&lt;br&gt; <br /> <br /> {| width=&quot;434&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | No.&lt;br&gt; <br /> | ID&lt;br&gt; <br /> | Plasmid Name&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | conc.(ug/ul) <br /> | TR (transfection reagent &lt;br&gt; <br /> | Color&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | 1353&lt;br&gt; <br /> | pCMV5-Fc(3C)-RFP-WPRE&lt;br&gt; <br /> | 2.0 <br /> | PEI&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | 1305&lt;br&gt; <br /> | pCMV5-Fc(3C)-TagRFP&lt;br&gt; <br /> | 1.0 <br /> | PEI&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | 1369&lt;br&gt; <br /> | pCMV5-Fc-(3c)-TagRFP-WPRE-oriP&lt;br&gt; <br /> | 2.8 <br /> | PEI&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | 174&lt;br&gt; <br /> | pLVXPlus2-IRES-mCherry &lt;br&gt; <br /> | 0.58 <br /> | PEI&lt;br&gt; <br /> | &amp;nbsp;a few&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | 529&lt;br&gt; <br /> | pLVX-mIL10-IRES-EGFP&lt;br&gt; <br /> | 2.8 <br /> | PEI&lt;br&gt; <br /> | weak&lt;br&gt;<br /> |}<br /> <br /> 12 well plate transfection:&lt;br&gt; use 1,2,3 ug DNA plasmid and 3 ul PEI TR . There is no RFP in clone 1353,1305 and 1369 . For clone 529, 2ug od DNA show weak GFP but not in 1ug and 3 ug at 16 hours. (transfected on 4:00PM and observed on 9:00AM next day)&lt;br&gt; <br /> <br /> Conclutsion: No show RFP expression. Compare to previous transfection results, 529 expression not as expected. maybe PEI&amp;nbsp;reagent are not very efficient. <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]] [[Category:QC]]</div> Xiuli.huang