https://eln.excellgen.com/index.php?action=history&feed=atom&title=1353-1305-1369_transfection_on_0913-2012 1353-1305-1369 transfection on 0913-2012 - Revision history 2026-10-18T05:56:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1353-1305-1369_transfection_on_0913-2012&diff=6072&oldid=prev Xiuli.huang at 16:28, 18 September 2012 2012-09-18T16:28:50Z <p></p> <p><b>New page</b></p><div>&lt;br&gt; <br /> <br /> {| width=&quot;434&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | No.&lt;br&gt; <br /> | ID&lt;br&gt; <br /> | Plasmid Name&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | conc.(ug/ul) <br /> | Lipofectanine 2000&lt;br&gt; <br /> | Roche &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | 1353&lt;br&gt; <br /> | pCMV5-Fc(3C)-RFP-WPRE&lt;br&gt; <br /> | 2.0 <br /> | &amp;nbsp; very weak red at 24hs&lt;br&gt; <br /> | no<br /> |-<br /> | 2&lt;br&gt; <br /> | 1305&lt;br&gt; <br /> | pCMV5-Fc(3C)-TagRFP&lt;br&gt; <br /> | 1.0 <br /> | very weak red at 24hs&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | 1369&lt;br&gt; <br /> | pCMV5-Fc-(3c)-TagRFP-WPRE-oriP&lt;br&gt; <br /> | 2.8 <br /> | very weak red at 24hs&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | 174&lt;br&gt; <br /> | pLVXPlus2-IRES-mCherry &lt;br&gt; <br /> | 0.58 <br /> | red&lt;br&gt; <br /> | less red&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | 529&lt;br&gt; <br /> | pLVX-mIL10-IRES-EGFP&lt;br&gt; <br /> | 2.8 <br /> | strong&lt;br&gt; <br /> | less strong&lt;br&gt;<br /> |}<br /> <br /> 12 well plate transfection (293F cells):&lt;br&gt; use 2 ug DNA plasmid and 6 ul lipofectamine and Roche transfection reagents . There is no RFP in clone 1353,1305 and 1369 at 16 hs, and start to show RFP at 24 hours. Cells started to die becase of the toxicity of lipo2000, For clone 529 and 174, transfection efficiency as expected.&lt;br&gt; <br /> <br /> Conclutsion: weak RFP expression with Lipofectamine 2000.&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]] [[Category:QC]]</div> Xiuli.huang