https://eln.excellgen.com/index.php?action=history&feed=atom&title=1353_pCMV5-Fc%283C%29-RFP-WPRE 1353 pCMV5-Fc(3C)-RFP-WPRE - Revision history 2026-10-18T14:06:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1353_pCMV5-Fc(3C)-RFP-WPRE&diff=6059&oldid=prev Paul.xu at 14:45, 18 September 2012 2012-09-18T14:45:50Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1353 pCMV5-Fc(3C)-RFP-WPRE&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1353 pCMV5-Fc(3C)-RFP-WPRE <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;August 21, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1353 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;August 21, 2012 <br /> <br /> Subclone RFP from 1305 pCMV5-Fc(3C)-TagRFP using Nhe1 + Not1 &lt;br&gt; <br /> <br /> [[Image:1353 Digestion.JPG]]&lt;br&gt; <br /> <br /> Digested insert 1305 pCMV5-Fc(3C)-TagRFP (lane 1) and vector 1336 (lane 2) with RE&amp;nbsp;Nhe1 +&amp;nbsp;Not1 &lt;br&gt; <br /> <br /> Cut out bands, purify and ligate together, transform <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> 2012-8-27&lt;br&gt; <br /> <br /> Nhe1 + Not1 &lt;br&gt; <br /> <br /> [[Image:1303+1335+1353 digest detct 8-27-2012.JPG]] <br /> <br /> 2012-9-14&lt;br&gt; <br /> <br /> [[Image:IMG_4211.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu