https://eln.excellgen.com/index.php?action=history&feed=atom&title=1356-pCMV5-Fc%283C%29-p53%281-342%29-SVLT
1356-pCMV5-Fc(3C)-p53(1-342)-SVLT - Revision history
2026-10-18T07:32:41Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=1356-pCMV5-Fc(3C)-p53(1-342)-SVLT&diff=5901&oldid=prev
Paul.xu at 18:31, 4 September 2012
2012-09-04T18:31:29Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1356-pCMV5-Fc(3C)-p53(1-342)-SVLT''' ==<br />
<br />
'''Purpose:''' Cloning of 1356-pCMV5-Fc(3C)-p53(1-342)-SVLT <br />
<br />
'''Date: '''2012-8-31---2012-9-4<br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:1356''' <br />
<br />
'''Experimenters:''' yingying <br />
<br />
== '''Results''' ==<br />
<br />
=== subcloning ===<br />
<br />
[[Image:1298+1324+1346+1347+1350+1356 clone 8-31-2012.JPG]]<br> <br />
<br />
subcloning from 1343 pDB.His.MBP-(3C)-p53 (1-342) t0 1224 pCMV5-Fc(3C)-Dest-SVLT using Not1 + Nhe1<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2012-9-4<br />
<br />
Make miniprep and digest with Not1 + Nhe1<br />
<br />
[[Image:1356+1358+1350_digest_detect_9-4-2012.JPG]]<br />
<br />
clone 1,2,3,4,5 may be right<br />
<br />
<br> <br />
<br />
<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
*&amp;amp;amp;lt;br&amp;amp;amp;gt;<br />
<br />
== '''Conclusion''' ==<br />
<br />
&amp;amp;amp;lt;br&amp;amp;amp;gt; <br />
<br />
{| cellspacing="1" cellpadding="1"<br />
|-<br />
| <br><br />
|}<br />
<br />
<br> <br />
<br />
{|<br />
|-<br />
| <br><br />
|}<br />
<br />
[[Category:Cloning]]</div>
Paul.xu