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136 1708 212 pL6H-Tat-Cre - Revision history
2026-10-18T14:45:03Z
Revision history for this page on the wiki
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Crm at 19:28, 9 October 2018
2018-10-09T19:28:47Z
<p></p>
<p><b>New page</b></p><div>Subcloning:<br />
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For 134: vector 212 and&nbsp; insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1<br />
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For 136: Vector 2038 and insert&nbsp; 129, Nco1 - Xho1&nbsp;<br />
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[[File:3257 212 1708 129 2038.JPG|RTENOTITLE]]<br />
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Miniprep digestion<br />
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[[File:134 136 135 3164 digestionJPG.jpeg|RTENOTITLE]]<br />
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Redo<br />
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10/7/2018<br />
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[[File:1708 212 3257.JPG|RTENOTITLE]]<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| Lane<br />
| ID<br />
| Name<br />
| Enzyme<br />
|-<br />
| 1<br />
| 129<br />
| <br/><br />
| Nde1-Not1<br />
|-<br />
| 2<br />
| 2038<br />
| <br/><br />
| Nde1-Not1<br/><br />
|-<br />
| 3<br />
| 1708<br />
| <br/><br />
| Nco1-Xho1<br />
|-<br />
| 4<br />
| 212<br />
| <br/><br />
| Nco1-Xho1<br/><br />
|-<br />
| 5<br />
| 3257<br />
| <br/><br />
| EcoR1-Nhe1<br />
|-<br />
| 6<br />
| 3260<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 7<br />
| 3261<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 8<br />
| 3259<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 9<br />
| 3258<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|-<br />
| 10<br />
| 3262<br />
| <br/><br />
| EcoR1-Nhe1<br/><br />
|}<br />
<br />
<br />
<br />
[[Category:Cloning]][[File:134 136 plasmid digestion.JPG]]</div>
Crm