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1372+1392 pilot transfection (ratio) - Revision history
2026-10-18T03:30:52Z
Revision history for this page on the wiki
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Paul.xu at 14:37, 4 October 2012
2012-10-04T14:37:43Z
<p></p>
<p><b>New page</b></p><div>[[Image:IMG 4237.JPG]] <br />
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{| width="307" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| 1392 (ug) <br />
| 0.05 <br />
| 0.1 <br />
| 0.2 <br />
| 0.4 <br />
| 0.5 <br />
| 1.0<br />
|-<br />
| 1372 (ug) <br />
| 0.5 <br />
| 0.5 <br />
| 0.5 <br />
| 0.5 <br />
| 0.5 <br />
| 1.0<br />
|}<br />
<br />
<br> <br />
<br />
12 well plate transfection (293T cells):<br> Purpose: want to check the stongest expression in the 1353, 1305, 1369 plasmid using 293T cells.<br> <br />
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[[Image:1372+1392 transfection 9-26-2012.jpg]]<br> <br />
<br />
1. 1372 (alone 0.5 ug) <br> <br />
<br />
2. 1372+1392 (0.05 ug) <br />
<br />
3. 1372+1392 (0.1 ug) <br />
<br />
4. 1372+1392 (0.2 ug) <br />
<br />
5. 1372+1392 (0.4 ug)&nbsp; ( can see dramatically (16 hour )better than 1372 alone and plus 1392 0.05 ug, 0.1 ug, 0.2 ug) <br />
<br />
0.1 ug and 0.2 ug, picture took later) <br />
<br />
6 1372+1392 (0.5 ug) <br />
<br />
7. 1372 (1 ug) + 1392 (1 ug) <br />
<br />
[[Category:Cloning]] [[Category:QC]]</div>
Paul.xu