https://eln.excellgen.com/index.php?action=history&feed=atom&title=1375-pDB.His.MBP.3C 2026-10-18T06:19:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1375-pDB.His.MBP.3C&diff=6352&oldid=prev 2012-10-07T23:24:36Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1375 pDB.His.MBP.3C&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; subcloning of 3C into pDB.His.MBP(V14) <br /> <br /> &lt;br&gt; &#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; 2012-09-18&lt;br&gt; <br /> <br /> &lt;br&gt; &#039;&#039;&#039;Notebook:&amp;amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1375&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: Xiuli&#039;&#039;&#039; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> Cloning <br /> <br /> [[Image:PDB.HIS 3C.jpg]] <br /> <br /> 09-19-2012 A.Restriction enzyme digestion: Vector V14 pDB.His.MBP and 1197 pDB.His.GST.3C with NdeI and XhoI, B.Gel purification (elute with 15 ul H2O), and ligation with T7 ligase at RT for2 hs (total volume in 30ul) C Transformation: DH5a + 20 ul ligation Re. <br /> <br /> There is no colony on the plate. re-do the experiment again. <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:9202012-1377-1381.jpg]] <br /> <br /> <br /> <br /> 10/7/2012<br /> <br /> Digest miniprep with Nde1 + Xho1<br /> <br /> [[Image:1375_10_7_2012.jpg]]<br /> <br /> clones 1,2, 4,5 look ok.<br /> <br /> [[Category:Cloning]]</div>

Paul.xu