https://eln.excellgen.com/index.php?action=history&feed=atom&title=1376-pCMV5-TPA-%283c%29-Fc-WPRE-OriP 2026-10-18T11:28:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1376-pCMV5-TPA-(3c)-Fc-WPRE-OriP&diff=6294&oldid=prev 2012-10-02T13:36:02Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1376-pCMV5-TPA-(3c)-Fc-WPRE-OriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1376-pCMV5-TPA-(3c)-Fc-WPRE-OriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-9-26---2012-9-29 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1376&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1376 PCR 9-26-2012.JPG]]&lt;br&gt; <br /> <br /> gene synthesis of ss and amplification of Fc from 1361 pCDM8-like-nSEBOV-delta-thr-Fc <br /> <br /> TPA-BamH1-F1, 1 ul of 10 uM&lt;br&gt;TPA-BamH1-F2 , 0.5 ul of 1 uM&lt;br&gt;TPA-BamH1-F3, 0.5 ul of 1 uM&lt;br&gt;TPA-BamH1-F4, 0.5 ul of 1 uM&lt;br&gt; <br /> <br /> Fc-6His-Eag1-R, 1 ul of 10 uM&lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> [[Image:1351+1376_clone_9-27-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | digest PCR product with BamH1 + Eag1, digest vector 1369 pCMV5-Fc-(3c)-TagRFP-WPRE-oriP with BamH1+ Eag1&lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012/9/29 <br /> <br /> 1376 digest with BamH1 + Eag1, size is about 1.5 kb <br /> <br /> [[Image:1376 1272 restrition digestion 9 29 2012.jpg]] <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

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