Paul.xu at 18:51, 2 October 2012

2012-10-02T18:51:22Z

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1377-1378-1379-1380-1380-1381 Plasmid Maxiprep 

 

Clones for maxiprep

9/24/2012

Select 1377-2, 1378-1, 1379-2, 1380-2, 1381-1 to make maxiprep using Qiagen maxiprep kit. 1381, 1392  using invitrogen maxiprep  kit

{| width="330" cellspacing="1" cellpadding="1" border="1"
|-
| Clone ID 
| Parent 
| Clone Name 
|
Expected Size

(bp) 

| Actual Size on Gel (Kb) 
| Clone Identity 
| DNA conc 
| OD260/280 
| RNA 
|-
| 1377-1 
| 1284 
| 
| 1647 
| 2.5 
| 
| 
| 
| 
|-
| 1378-2 
| 1276 
| 
| 1322 
| 1.6 
| 
| 
| 
| 
|-
| 1379-2 
| 1264 
| 
| 3205 
| 4 
| 
| 
| 
| 
|-
| 1380-2 
| 1269 
| 
| 2169 
| 3 
| 
| 
| 
| 
|-
| 1381-1 
| 1263 
| 
| 1586 
| 2.5 
| 
| 
| 
| 
|}

 [[Image:1377-1381 maxi cut .jpg]][[Image:1377-1381 maxi-cut.jpg]]

 

 

1381 and 1392 using Invitrogen maxi prep kit have too much RNA. After treating  sample 1381 and 1392 with 0.5 ul RNAse (Qiagen) at 37oC , After 2-3 hours, there are still too much RNA on the gel, adding 10 ul (100mg/ml RNAse) into the sample and incubate at 37C for another 4-6 hours, precipitate DNA with isoprapanol. There isn't pure (Ratio is low), so the DNA reading is very low (minus value).There is too much RNase protein. Paul run the sample on the qiagen column again and there is no too much DNA left. Need re-do maxipre for this two sample. 

[[Category:QC]]

1377-1378-1379-1380-1380-1381 Plasmid - Revision history

Paul.xu at 18:51, 2 October 2012