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1382-1406 Purification on 11-7-2012 - Revision history
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Paul.xu at 19:58, 8 November 2012
2012-11-08T19:58:29Z
<p></p>
<p><b>New page</b></p><div>'''1382-1406 purification on 11-07-2012 by Xiuli '''<br> <br />
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Cell Viability: 1386 has less cells and poor viability, <br> <br />
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Collect 30 ml Serum-free medium from each flasks (5)&nbsp;and and 1 plate (1382) 20 ml and centriguged at 12000rpm for 15 mins. <br />
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Incubate supernanats with protein A beads, washed with PBS and elute with 0.2 mM Glycine (pH=2.0), elution volume:300 ul <br />
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Predicted expressed protein size<br> <br />
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{| width="421" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| 1382 <br />
| 1383 <br />
| 1385 <br />
| 1386 <br />
| 1406<br />
|-<br />
| 110KD <br />
| 48-54KD <br />
| 79KD <br />
| 60KD <br />
| 61KD <br />
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|}<br />
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Load 20-35 ul on the 4-20% SDS gel <br />
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{| width="421" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| <br> <br />
| 1382 <br />
| 1383 <br />
| 1385 <br />
| 1386 <br />
| 1406 <br />
| 1386<br />
|-<br />
| Lane <br> <br />
| 1,2,3<br> <br />
| 4,5,6 <br />
| 7,8,9, <br />
| 10,11,12 <br />
| 13,14,15, <br />
| 16 <br />
<br> <br />
<br />
|}<br />
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[[Image:1382-1406 11-7-2012.jpg]] <br />
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The protein size is not the predicted size except 1383.<br> <br />
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[[Category:Protein_Purification]]</div>
Paul.xu