https://eln.excellgen.com/index.php?action=history&feed=atom&title=1386_pCMV5-TPA-RIPK3-%283c%29-Fc-WPRE-oriP 1386 pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP - Revision history 2026-10-18T05:14:02Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1386_pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP&diff=6450&oldid=prev Paul.xu at 11:55, 11 October 2012 2012-10-11T11:55:46Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1386 pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1386 pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date:&amp;nbsp;2012-9-29 ~&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:&amp;nbsp;1396&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul &amp;amp; Shanyi &amp;amp; Xiuli&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> 2012-9-29 <br /> <br /> [[Image:PCR 9 29 2012.jpg]]&lt;br&gt; <br /> <br /> 2012/9/30 digestion: &lt;br&gt; <br /> <br /> {| width=&quot;200&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | Name&lt;br&gt; <br /> | TagGFP&lt;br&gt; <br /> | 1369&lt;br&gt; <br /> | 1376&lt;br&gt; <br /> | TagGFP&lt;br&gt;<br /> |-<br /> | Digestion&lt;br&gt; <br /> | Nhe1+Not1&lt;br&gt; <br /> | Nhe1+Not1&lt;br&gt; <br /> | Nhe1 + Kpn1&lt;br&gt; <br /> | Nhe1 + Kpn1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1407 139 digestion 2012 9 30.jpg]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 10/7/2012&lt;br&gt; <br /> <br /> [[Image:1385 1386 1406 10 7 2012.jpg]] <br /> <br /> clone #3 &amp;amp; 4 may be ok <br /> <br /> 10-08-2012 <br /> <br /> Re-screening colony with PCR methods. #7 #8 in 1386 below is the same clone #3 and #4 above miniprep. &lt;br&gt; <br /> <br /> [[Image:1385-1386 PCR screening.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1386-1406 screening.JPG]]&lt;br&gt; <br /> <br /> 1, 3,&amp;nbsp; 4, 5, 6 looks right size. but #7 and #8 didn&#039;t work.( it&#039;s positive clone #3 and #4 in miniprep screening.&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu