https://eln.excellgen.com/index.php?action=history&feed=atom&title=1400_pCMV5-Gag-NLS-Cre-WPRE-oriP 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP - Revision history 2026-10-18T12:53:20Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1400_pCMV5-Gag-NLS-Cre-WPRE-oriP&diff=10420&oldid=prev Paul.xu at 13:58, 17 October 2013 2013-10-17T13:58:25Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-11-05--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1400&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:V16 pCR2.1AND 1293P V15 414 1449 1445P 1449 11 05 2012.JPG]]&lt;br&gt; <br /> <br /> subclone cre from 414 to 1449, use EcoRI&amp;amp;NotI DIGEST.&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-11-7 <br /> <br /> Make miniprep and digest with EcoRI&amp;amp;NotI <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1400digest11 07-2012.JPG]] <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Cloning of 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1400 pCMV5-Gag-NLS-Cre-WPRE-oriP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-15--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1400&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone cre from 414 to 1449, use EcoRI&amp;amp;NotI DIGEST.&lt;br&gt; <br /> <br /> [[Image:DIGEST_FOR1400.JPG‎ ]]<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-11-7 <br /> <br /> Make miniprep&amp;nbsp; <br /> <br /> [[Image:1400DIGEST.JPG‎ ]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu