https://eln.excellgen.com/index.php?action=history&feed=atom&title=1402-TurboGFP 1402-TurboGFP - Revision history 2026-10-18T07:24:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1402-TurboGFP&diff=6248&oldid=prev Paul.xu at 17:53, 27 September 2012 2012-09-27T17:53:49Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1402- pLVX-TurboGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1402- pLVX-TurboGFP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-9-25---2012-9-27 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1402&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR the same with 1272 clone&lt;br&gt; <br /> <br /> [[Image:1272 PCR 9-21-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:559+567+1402+1352 clone 9-24-2012.JPG]]&lt;br&gt; <br /> <br /> (1272) lane 5: PCR product&lt;br&gt; <br /> <br /> &amp;nbsp; lane 6: plasmid 46,pLVX-TagGFP-2&lt;br&gt; <br /> <br /> &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xho1+Xba1 <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-9-27&lt;br&gt; <br /> <br /> mini prep and digest with Xho1+Xba1 &lt;br&gt;<br /> <br /> [[Image:1106+1402_digest_detect_9-27-2012.JPG]]&lt;br&gt;<br /> <br /> clone 1,2,3,4 may be right<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu