https://eln.excellgen.com/index.php?action=history&feed=atom&title=1403-pCDNA3.1-NLS-Cre 1403-pCDNA3.1-NLS-Cre - Revision history 2026-10-18T07:27:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1403-pCDNA3.1-NLS-Cre&diff=11957&oldid=prev Paul.xu at 15:34, 10 June 2014 2014-06-10T15:34:11Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1403-pCDNA3.1-NLS-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1403-pCDNA3.1-NLS-Cre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-9-25---2012-9-27 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1403&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1246+1403 clone 9-25-2012.JPG]]&lt;br&gt; <br /> <br /> subclone from 241 pLentiGP-Cre using EcoR1 + Xho1 to 1226 pCDNA3.1+&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-9-27 <br /> <br /> mini prep and digest with EcoR1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1403 digest detect 9-27-2012.JPG]][[Image:1403 digest detect 9-27-2-2012.JPG]][[Image:1403 digest detect 9-28-2012.JPG]]&lt;br&gt; <br /> <br /> clone 3 and 6 are right <br /> <br /> 1403 digest with EcoRI+XhoI&lt;br&gt; <br /> <br /> [[Image:1403digest1.JPG]]&lt;br&gt; <br /> <br /> 1403DIGEST WITH DraIII +EcoRI(1), EcoRI+XbaI(2)&lt;br&gt; <br /> <br /> 1403DIGEST WITH DraIII +EcoRI, should get&amp;nbsp; two bands, if it is 241, should get there bands.&lt;br&gt; <br /> <br /> 1403DIGEST WITH EcoRI+XbaI,should get&amp;nbsp; two bands, if it is 241,only one.&lt;br&gt; <br /> <br /> [[Image:1403digest2.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1403-pCDNA3.1-NLS-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1403-pCDNA3.1-NLS-Cre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1403&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; shanyi<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning&lt;br&gt; ===<br /> <br /> subclone from 241 pLentiGP-Cre using EcoR1 + Xho1 to 1226 pCDNA3.1+&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | [[Image:Digest_for_2110_1403.JPG]]&lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6<br /> <br /> mini prep and digest with EcoR1 + Xho1 <br /> <br /> [[Image:1403_2110digest.JPG]]<br /> <br /> verify<br /> <br /> [[Image:1403DIGEST.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu