https://eln.excellgen.com/index.php?action=history&feed=atom&title=1406-pCMV5-TPA-NPR1-Cyto-Fc%283c%29-WPRE-OriP 1406-pCMV5-TPA-NPR1-Cyto-Fc(3c)-WPRE-OriP - Revision history 2026-10-18T09:57:15Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1406-pCMV5-TPA-NPR1-Cyto-Fc(3c)-WPRE-OriP&diff=6451&oldid=prev Paul.xu at 11:58, 11 October 2012 2012-10-11T11:58:57Z <p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Cloning of 1406-&#039;&#039;&#039;pCMV5-TPA-NPR1-Cyto-Fc(3c)-WPRE-OriP <br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1406 pCMV5-TPA-NPR1-Cyto-Fc(3c)-WPRE-OriP <br /> <br /> &#039;&#039;&#039;Date:&amp;amp;nbsp;2012-9-29 ~&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:&amp;amp;nbsp;1406&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul &amp;amp;amp; Shanyi &amp;amp;amp; Xiuli&amp;lt;bjavascript:void(0)r&amp;gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === PCR ===<br /> <br /> 2012-9-29--ShanYi <br /> <br /> [[Image:PCR 9 29 2012.jpg]] <br /> <br /> &amp;nbsp;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> Date: 10/7/2012&lt;br&gt; <br /> <br /> [[Image:1385 1386 1406 10 7 2012.jpg]] <br /> <br /> clone #1 maybe ok <br /> <br /> &lt;br&gt; <br /> <br /> 10-08-2012 <br /> <br /> Re-screening clony with PCR.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> No 8 in 1406 below is the same positive #1 clone in miniprep&lt;br&gt; <br /> <br /> [[Image:1386-1406 screening.JPG]]&lt;br&gt; <br /> <br /> All colony except # 4 screened looks right size band (1.6kb) <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu