https://eln.excellgen.com/index.php?action=history&feed=atom&title=1413_pCDNA3.4-Rabbit-IgG_Heavy_Chain 2026-10-18T11:34:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1413_pCDNA3.4-Rabbit-IgG_Heavy_Chain&diff=6367&oldid=prev 2012-10-09T13:02:33Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1413 pCDNA3.4-Rabbit-IgG Heavy Chain&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1413 pCDNA3.4-Rabbit-IgG Heavy Chain <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-10-3 ~2012-9-5<br /> <br /> &#039;&#039;&#039;Notebook: &#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1413 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning &lt;br&gt; ===<br /> <br /> Date:10-3-2012 <br /> <br /> Digest 1 ug plasmid DNA 1410 pCDNA3.4-Rabbit-IgG H+L with Xba1 + Age1&lt;br&gt; <br /> <br /> [[Image:1410 digest 10-3-2012.JPG]] <br /> <br /> cut out H &amp;amp; L, also vector. Ligate and transform DH5alpha <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> 2012-9-5&lt;br&gt;<br /> <br /> mini prep and digest with Xba1 + Age1<br /> <br /> [[Image:1413+1414+1324_digest_detect_10-5-2012.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu